Asian Journal of Research in Chemistry

Subash Chandra Verma ¹, Rachana Rani ¹, Sukriti Nigam ¹, Dinesh Kumar Agrawala ¹, Pramila Pant ¹, Madan Mohan Padhi ¹, Ramesh Babu Devalla ¹, Chhoten Lal Jain ²

Affiliations
1 Central Council for Research in Ayurveda and Siddha, 61-65, Institutional Area, Opp.-D-Block, Janakpuri, New Delhi-110058, IN
2 Department of Chemistry, M. M. H. College, Ghaziabad, U.P., IN

Abstract

Quality assurance of herbal products may be ensured by proper quality control of the herbal ingredients. In this regard, a rapid, precise, selective, and accurate high performance thin layer chromatographic method of analysis for the determination of ferulic acid in Hemidesmus indicus ischolar_mains extract was developed and validated. The method employed, TLC aluminium plates pre-coated with silica gel 60 F₂₅₄ as the stationary phase. The mobile phase consisted of Toluene: Ethyl acetate: Formic acid; 5:5:0.2 (v/v/v). This method was found to give compact spots for ferulic acid (R_f value of 0.59 ± 0.01). Densitometric scanning of ferulic acid was carried out at λ366 nm in the absorbance/reflection mode. The linear regression analysis data for the calibration plots showed good linear relationship (r²) = 0.999 with respect to peak area and in the concentration range of 50-300 ng spot^-1 band. The method was validated for linearity, precision, limit of detection and limit of quantitation, accuracy and recovery. The present study signifies the use of TLC, HPTLC fingerprint profiles for determining the identity, purity and strength of the ferulic acid in the H. indicus ischolar_main and also for fixing standards for H. indicus containing Ayurvedic formulation.

Keywords

Hemidesmus indicus, HPTLC Method, Development and Validation, Ferulic Acid, Quality Control.

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